Anti-Caveolin 1 Antibody

• Catalog Number: orb213667
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Caveolin 1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caveolin 1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CAV1
• Entrez: 857, 12389
• UniProt ID: P49817, Q03135, P41350
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti BSCL3 antibody; anti CAV 1 antibody; anti CAV
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Caveolin 1 expression in Hela (A), H1688 (B), mouse lung (C), rat lung (D) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 23; 25 kD)

Immunofluorescent analysis of Caveolin 1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).