Caveolin-1 Rabbit Polyclonal Antibody

• Catalog Number: orb10244
• Category: Antibodies
• Description: Caveolin-1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Caveolin-1 (2-120/178aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 20 kDa
• Target: CAV1
• UniProt ID: Q03135
• RRID: AB_10750358
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: opamine D3 receptor; Caveolin1; Caveolin 1; 3 dopa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Cheng, Mao-Feng et al. The role of rosiglitazone in the proliferation of vascular smooth muscle cells after experimental subarachnoid hemorrhage Acta Neurochir (Wien), 156, 2103-2109 (2014)

PubMed: 25139403

Paraformaldehyde-fixed, paraffin embedded (Rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caveolin-1) Polyclonal Antibody, Unconjugated (orb10244) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: Lung (Mouse) Lysate at 40 ug, Bladder (Mouse) Lysate at 40 ug, Primary: Anti-Caveolin-1 (orb10244) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-Caveolin-1 (orb10244) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD.

Sample: NIH/3T3 Cell Lysate at 40 ug, A549 Cell Lysate at 40 ug, HUVEC Cell Lysate at 40 ug, Primary: Anti-Caveolin-1 (orb10244) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD.

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (orb10244) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: mouse retina tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (orb10244) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.