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| Catalog Number | orb770517 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to CaMKII alpha/delta (phospho-Thr286) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Porcine, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CaMK2 around the phosphorylation site of Thr286. AA range:256-305 |
| MW | 54 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-CAMK2A antibody, anti-CAMKA antibody, anti-KI Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Western Blot analysis of HELA cells using Phospho-CaMKIIα/δ (T286) Polyclonal Antibody diluted at 1:500.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK2 (Phospho-Thr286) Antibody.

Immunofluorescence analysis of COS7 cells, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.