CA125/MUC16 Rabbit Monoclonal Antibody

SKU: orb547818

Description

Anti-CA125 / MUC16 Monoclonal Antibody. Tested in WB, IHC applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cell Biology, Disease Biomarkers, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AFFO-13
• Immunogen: A synthesized peptide derived from human CA-125 (MUC16) Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
• Target: Mucin-16
• Molecular Weight: 80 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mucin-16; MUC-16; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125; MUC16; CA125

IHC analysis of MUC16 using anti-MUC16 antibody (orb547818). MUC16 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MUC16 Antibody (orb547818) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Quick Database Links

Gene Symbol

Mucin-16

UniProt

Q8WXI7