MCM7 Monoclonal Antibody

SKU: orb547820

Description

Anti-MCM7 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

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Tested Applications	FC, ICC, IF, IHC, IP, WB
Reactivity	Human, Mouse, Rat
Application Notes	WB 1:500-2000IHC 1:50-200ICC/IF 1:50-200IP 1:20FC 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	BA-13
Immunogen	A synthesized peptide derived from human MCM7 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit.
Molecular Weight	79 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Concentration	0.5mg/ml
Disclaimer	For research use only

Alternative Names

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Mucin-16; MUC-16; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125; MUC16; CA125

Quality Guarantee

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Western blot analysis of MCM7 expression in HeLa cell lysate using anti-MCM7 antibody (orb547820). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified polyclonal antibody (Catalog # orb547820) at 0.5 ug/mL overnight at 4.