Anti-C3a-receptor [3G7]

SKU: orb348843

Description

Mouse monoclonal antibody to C3a-receptor

Images & Validation

• Tested Applications: ELISA, FC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3G7
• Immunogen: The 2e loop of the C3a receptor.
• Target: C3a-receptor
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Complement component receptor 1; C3AR1

Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-C3a-receptor antibody 3G7. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3G7 (orb348879) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb348879, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

C3a-receptor

UniProt

Q16581

References

Hawlisch, H. et al. Site-specific anti-C3a receptor single-chain antibodies selected by differential panning on cellulose sheets Anal Biochem, 293, 142-145 (2001)

PubMed: 11373092