Anti-C3a-receptor [3G7]

• Catalog Number: orb348879
• Category: Antibodies
• Description: Rabbit monoclonal antibody to C3a-receptor
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3G7
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: The 2e loop of the C3a receptor.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: C3a-receptor
• UniProt ID: Q16581
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Complement component receptor 1; C3AR1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hawlisch, H. et al. Site-specific anti-C3a receptor single-chain antibodies selected by differential panning on cellulose sheets Anal Biochem, 293, 142-145 (2001)

PubMed: 11373092

Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-C3a-receptor antibody 3G7. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3G7 (orb348879) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb348879, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.