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Anti-BID Antibody

Catalog Number: orb1473850

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1473850
CategoryAntibodies
DescriptionMouse monoclonal antibody to BID
Species/HostMouse
ClonalityMonoclonal
Tested applicationsIF, IH, WB
ReactivityHuman
ImmunogenRecombinant fusion protein of human BID. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)
Form/AppearanceMouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetBID
Entrez637
UniProt IDP55957
SourceMouse
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesMouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesBH3-interacting domain death agonist; p22 BID; BID
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-BID Antibody

Western blot analysis of BID expression in HeLa (A), A431 (B), MDAMB231 (C), Raji (D), Jurkat (E), THP1 (F) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 22 kD)

Anti-BID Antibody

Immunohistochemical analysis of BID staining in human bladder formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-BID Antibody

Immunofluorescent analysis of BID staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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    IF,  IH,  WB

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