Beta1-tubulin Mouse Monoclonal Antibody

SKU: orb323098

Description

The Beta1-tubulin Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human Beta1-tubulin. The exact sequence is proprietary. Purification: Affinity chromatography

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:5000-1:10000, IHC-P: 1:200-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta1-tubulin. The exact sequence is proprietary.
• Target: TUBB1
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tubulin beta-1 chain

Western blot analysis of Beta1-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 52 kD)

Immunohistochemical analysis of Beta1-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

TUBB1

UniProt

A2AQ07, Q9H4B7

NCBI Gene ID

545486, 81027