Bcl-2 Rabbit Monoclonal Antibody

SKU: orb547002

Description

Anti-Bcl-2 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Dilution Range: WB 1:500-1:2000 IHC 1:50-1:100 ICC/IF 1:50-1:100 IP 1:50 FC 1:50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: HF-2
• Immunogen: A synthesized peptide derived from human Bcl-2
• Target: Apoptosis regulator Bcl-2
• Molecular Weight: 83, 88 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis regulator Bcl-2; BCL2

WB analysis of Bcl-2 expression in (1) Jurkat Cell;(2) MCF-7 Cell.

Immunofluorescent analysis of Jurkat cells, using Bcl-2 Antibody

Immunohistochemical analysis of paraffin-embedded human spleen, using Bcl-2 Antibody.

Quick Database Links

Gene Symbol

Apoptosis regulator Bcl-2

UniProt

P10415