B7-H1/PD-L1/CD274 Antibody

SKU: orb1806318

Description

Anti-B7-H1 / PD-L1 / CD274 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hulambda. It has a predicted MW of 145.5 kDa.

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Canine, Human, Monkey, Mouse, Rabbit, Rat

Key Properties

• Clonality: Monoclonal
• Target: B7-H1 / PD-L1 / CD274
• Source/Expression System: CHO
• Molecular Weight: 150 kDa
• Purity: >95%
• Purification: Protein A
• Endotoxin: <0.001 EU/ug

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Concentration: Batch dependent
• Disclaimer: For research use only

Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (avelumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (avelumab)is more than 98.22% ;determined by SEC-HPLC.

Immobilized human PD L1 FC at 2 μg/mL can bind Anti-B7-H1 / PD-L1 / CD274 Reference Antibody (avelumab);EC50=0.03725μg/mL.

Avelumab inhibited the tumor growth of MC38 on hLAG3 mice. The result showed significant anti-tumor effects; with an tumor inhibition rate (TGI) of 32.6% at 1 mpk at D28.

Quick Database Links

Gene Symbol

B7-H1 / PD-L1 / CD274

UniProt

Q9NZQ7