Anti-B7-2 antibody(DM84); Rabbit mAb

• Catalog Number: orb757406
• Category: Antibodies
• Description: Anti-B7-2 antibody(DM84), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM84
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: B7-2
• UniProt ID: P42081
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD86, B7-2, B70, CD28LG2, LAB72, MGC34413
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2 protein, mFc-His tagged protein orb689395 can bind Rabbit anti-B7-2 monoclonal antibody (clone: DM84) in a linear range of 1-100 ng/ml.

Expi 293 cell line transfected with irrelevant protein (A) and human B7-2 (B) were surface stained with Rabbit anti-B7-2 monoclonal antibody 1 μg/ml (clone: DM84) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry analysis of antigen binding of rabbit anti-human B7-2 mAb (orb757406). (A) orb757406 does not bind to 293T cells that do not express B7-2. (B) A clear peak shift of orb757406 was seen compared to the control when incubated with B7-2-expressing Daudi cells, indicating strong binding of orb757406 to B7-2. Antibodies were incubated at 5 μg/mL.

Flow cytometry analysis of antigen binding of rabbit anti-human B7-2 mAb (orb757406). (A) orb757406 does not bind to 293T cells that do not express B7-2. (B) A clear peak shift of orb757406 was seen compared to the control when incubated with B7-2-expressing Raji cells, indicating strong binding of orb757406 to B7-2. Antibodies were incubated at 5 μg/mL.