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Catalog Number | orb665550 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to ATRX |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATRX. The exact sequence is proprietary. |
Antibody Type | Primary Antibody |
Dilution range | WB: 1-500-1000, IF: 1-100-500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | ATRX |
Entrez | 546, 22589 |
UniProt ID | Q61687, P46100, P70486 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Anti-RAD54L antibody, anti-XH2 antibody, anti-Tran Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ATRX expression in 3T3L1 (A), C6 (B), SGC7901 (C), MCF7 (D), EC9706 (E) whole cell lysates. (Predicted band size: 282 kD; Observed band size: 285 kD)
Immunofluorescent analysis of ATRX staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |