Anti-ATG4A Antibody

• Catalog Number: orb1473718
• Category: Antibodies
• Description: Mouse monoclonal antibody to ATG4A
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IF, IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human ATG4A. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ATG4A
• Entrez: 115201
• UniProt ID: Q8WYN0
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: APG4A; AUTL2; Cysteine protease ATG4A; AUT-like 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATG4A expression in K562 (A) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 45 kD)

Immunohistochemical analysis of ATG4A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of ATG4A staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of Hela cells using Anti-ATG4A Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.