Atg4A Rabbit Monoclonal Antibody

SKU: orb548419

Description

Anti-Atg4A Monoclonal Antibody. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200 IP 1:20
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: BCEJDX3
• Immunogen: A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.
• Target: Cysteine protease ATG4A
• Molecular Weight: 45 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Death domain-containing protein CRADD; Caspase and RIP adapter with death domain; RIP-associated protein with a death domain; CRADD; RAIDD

Quick Database Links

Gene Symbol

Cysteine protease ATG4A

UniProt

Q8WYN0