ARF6 Rabbit Polyclonal Antibody

SKU: orb213580

Description

The ARF6 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARF6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-1-1000
• Reactivity: Gallus, Human, Mouse, Porcine, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARF6. The exact sequence is proprietary.
• Target: ARF6
• Molecular Weight: 20 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADP-ribosylation factor 6

Immunofluorescent analysis of ARF6 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Immunohistochemical analysis of paraffin-embedded human liver cancer using ARF6 Antibody orb213580 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse heart using ARF6 Antibody orb213580 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat lung using ARF6 Antibody orb213580 with dilution at 1:100.

Western blot analysis of Hela (1), rat liver (2) using ARF6 Antibody orb213580 with dilution at 1:1000.

Quick Database Links

Gene Symbol

ARF6

UniProt

P62330, P62331, P62332

NCBI Gene ID

382, 11845, 79121