APTX Rabbit Polyclonal Antibody

SKU: orb377929

Description

The APTX Antibody is suitable for IF, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human APTX .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, DNA Damage, Epigenetics, Neuroscience

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human APTX
• Target: APTX
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AXA1; Aprataxin; Forkhead-associated domain histidine triad-like protein; FHA-HIT

Western blot analysis of APTX expression in A549 (A), K562 (B), Raji (C) whole cell lysates. (Predicted band size: 5; 13; 19-40 kD; Observed band size: 40 kD)

Immunofluorescent analysis of APTX staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

APTX

UniProt

Q8K4H4, Q7Z2E3, Q7TQC5

NCBI Gene ID

54840, 66408, 259271