ANO1 Rabbit Polyclonal Antibody

SKU: orb705623

Description

Rabbit polyclonal antibody to ANO1

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-1:2000, IF: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human ANO1. The exact sequence is proprietary.
• Target: ANO1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-DOG1 antibody, anti-ORAOV2 antibody, anti-TAOS2 antibody, anti-TMEM16A antibody, anti-Anoctamin-1 antibody, anti-Discovered on gastrointestinal stromal tumors protein 1 antibody, anti-Oral cancer overexpressed protein 2 antibody, anti-Transmembrane protein 16A antibody, anti-Tumor-amplified and overexpressed sequence 2 antibody

Western blot analysis of ANO1 expression in LO2 (A), HeLa (B), mouse lung (C), rat skeletal muscle (D) whole cell lysates. (Predicted band size: 74; 97; 114 kD; Observed band size: 114 kD)

Immunofluorescent analysis of ANO1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

ANO1

UniProt

Q8BHY3, Q5XXA6

NCBI Gene ID

55107, 101772