AMPK alpha 2 Rabbit Polyclonal Antibody

SKU: orb412587

Description

The AMPK alpha 2 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human AMPK alpha 2 .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Autophagic, Cancer, Epigenetics, Neuroscience, Signaling Pathways, Wnt

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-2000, IF/ICC: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human AMPK alpha 2
• Target: PRKAA2
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase

Western blot analysis of AMPK alpha 2 expression in SW620 (A), mouse brain (B), rat heart (C) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 62 kD)

Immunofluorescent analysis of AMPK alpha 2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

PRKAA2

UniProt

P54646, Q8BRK8, Q09137

NCBI Gene ID

5563, 108079, 78975