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Anti-AMPK alpha 1 Antibody

Catalog Number: orb338932

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb338932
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PRKAA1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AMPK alpha 1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPRKAA1
Entrez5562, 65248, 105787
UniProt IDQ5EG47, Q13131, P54645
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti AMPK1 antibody, anti 5'-AMP-activated protein
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-AMPK alpha 1 Antibody

Western blot analysis of AMPK alpha 1 expression in Hela (A), HEK293T (B), rat spleen (C) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 63 kD)

Anti-AMPK alpha 1 Antibody

Immunohistochemical analysis of AMPK alpha 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-AMPK alpha 1 Antibody

Immunofluorescent analysis of AMPK alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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