Anti-AMPK alpha 1 Antibody

• Catalog Number: orb323094
• Category: Antibodies
• Description: Mouse monoclonal antibody to AMPK alpha 1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human AMPK alpha 1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:100-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKAA1
• Entrez: 5562
• UniProt ID: Q13131
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti AMPK1 antibody, anti 5'-AMP-activated protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMPK alpha 1 expression in Hela (A), 293T (B), NIH3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 64 kD)

Immunohistochemical analysis of AMPK alpha 1 staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.