AMPK alpha 1 Mouse Monoclonal Antibody

SKU: orb1473794

Description

The AMPK alpha 1 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human AMPK alpha 1. The exact sequence is proprietary.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant fusion protein of human AMPK alpha 1. The exact sequence is proprietary.
• Target: PRKAA1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: crude ascites with 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1

Western blot analysis of AMPK alpha 1 expression in MCF7 (A), MDAMB468 (B) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 64 kD)

Immunohistochemical analysis of AMPK alpha 1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

PRKAA1

UniProt

Q13131

NCBI Gene ID

5562