Anti-AMPK alpha 1 Antibody

• Catalog Number: orb1473794
• Category: Antibodies
• Description: Mouse monoclonal antibody to AMPK alpha 1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human AMPK alpha 1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Mouse IgG1 kappa. Supplied in crude ascites with 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKAA1
• Entrez: 5562
• UniProt ID: Q13131
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Supplied in crude ascites with 0.01% sodium azide.
• Alternative names: AMPK1; 5'-AMP-activated protein kinase catalytic s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMPK alpha 1 expression in MCF7 (A), MDAMB468 (B) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 64 kD)

Immunohistochemical analysis of AMPK alpha 1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.