Anti-alpha Smooth Muscle Actin Antibody

• Catalog Number: orb705521
• Category: Antibodies
• Description: Mouse monoclonal antibody to ACTA2
• Target: ACTA2
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human alpha Smooth Muscle Actin. The exact sequence is proprietary.
• UniProt ID: P62736
• Tested applications: IF, IH
• Dilution range: IHC-P: 1:100-1:300
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-ACTSA antibody, anti-ACTVS antibody, anti-Act
• Note: For research use only
• Entrez: 59

Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human cardiac muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human liomyoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.