AKT2 Mouse Monoclonal Antibody

SKU: orb1473916

Description

The AKT2 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human AKT2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/500 - 1/1000)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: Recombinant fusion protein of human AKT2. The exact sequence is proprietary.
• Target: AKT2
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta

Western blot analysis of AKT2 expression in Jurkat (A), Hela (B), mouse brain (C), C6 (D) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 56 kD)

Immunofluorescent analysis of AKT2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

AKT2

UniProt

P47197, Q60823, P31751

NCBI Gene ID

208, 11652, 25233