Anti-AHRR Antibody

• Catalog Number: orb1804656
• Category: Antibodies
• Description: The aryl-hydrocarbon receptor repressor also known as AHRR is a human gene. The protein encoded by this gene participates in the aryl hydrocarbon receptor (AhR) signaling cascade, which mediates dioxin toxicity, and is involved in regulation of cell growth and differentiation. It functions as a feedback modulator by repressing AhR-dependent gene expression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Immunogen: E.coli-derived human AHRR recombinant protein (Position: K410-D665)
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry, 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: Observed Molecular Weight: 100 kDa
• Target: Reactive to AHRR in Human
• UniProt ID: A9YTQ3
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HEL cells using anti-AHRR antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of AHRR using anti-AHRR antibody.Lane 1:human THP-1 cell;2:human HeLa cell;3:human SiHa cell;4:human HEL cell.

IF analysis of AHRR using anti-AHRR antibody and anti-Beta Tubulin antibody. AHRR was detected in immunocytochemical section of HELA cell.

IHC analysis of AHRR using anti-AHRR antibody. AHRR was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue.

IHC analysis of AHRR using anti-AHRR antibody. AHRR was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.

IHC analysis of AHRR using anti-AHRR antibody. AHRR was detected in a paraffin-embedded section of human glioma tissue.

IHC analysis of AHRR using anti-AHRR antibody. AHRR was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of AHRR using anti-AHRR antibody. AHRR was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of AHRR using anti-AHRR antibody. AHRR was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of AHRR using anti-AHRR antibody. AHRR was detected in a paraffin-embedded section of human spleen tissue.