Anti-Adenosine A3 Receptor Antibody

• Catalog Number: orb393197
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ADORA3.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Adenosine A3 Receptor. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500:1000, IF/ICC: 1:100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ADORA3
• Entrez: 25370, 100911796, 140
• UniProt ID: P0DMS8, P28647, Q61618
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Adenosine receptor A3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Adenosine A3 Receptor expression in MCF7 (A), H1792 (B) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 44; 66 kD)

Immunofluorescent analysis of Adenosine A3 Receptor staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).