Adenosine A3 Receptor Rabbit Polyclonal Antibody

• Catalog Number: orb10053
• Category: Antibodies
• Description: Adenosine A3 Receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADORA3 (85-180/347aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: ADORA3
• UniProt ID: P0DMS8
• RRID: AB_10751536
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: adenosine A3 receptor; A3AR; AD 026; AD026; ADORA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Raji (blue) (The cells were fixed with 70% ethanol overnight at -20℃). Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody (orb10053), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test.

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody (orb10053), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (orb10053) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Heart (Mouse) Tissue Lysate at 40 ug, Lane 2: Bone (Mouse) Tissue Lysate at 40 ug, Lane 3: Heart (Rat) Tissue Lysate at 40 ug, Primary: Anti-Adenosine A3 Receptor (orb10053) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 36 kD.

Tissue/cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated (orb10053) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.