Anti-Adenosine A2a Receptor Antibody

• Catalog Number: orb338920
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ADORA2A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Adenosine A2a Receptor. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ADORA2A
• Entrez: 135, 25369, 11540
• UniProt ID: P29274, P30543, Q60613
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti ADORA2 antibody, anti Adenosine receptor A2a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Seydyousefi, Mehdi et al. Exogenous adenosine facilitates neuroprotection and functional recovery following cerebral ischemia in rats Brain Res. Bull., 153, 250-256 (2019)

PubMed: 31545998

Applications

IHC

Reactivity

Rat

Guo, Fangming et al. Shenfu Administration Improves Cardiac Fibrosis in Rats With Myocardial Ischemia-Reperfusion Through Adenosine A2a Receptor Activation Hum Exp Toxicol, 41, (2022)

PubMed: 35196174

Applications

IHC

Reactivity

Rat

Western blot analysis of Adenosine A2a Receptor expression in Hela (A), HepG2 (B), NIH3T3 (C), H9C2 (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 45 kD)

Immunohistochemical analysis of Adenosine A2a Receptor staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Adenosine A2a Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.