Adenosine Receptor A2a Rabbit Polyclonal Antibody

• Catalog Number: orb10012
• Category: Antibodies
• Description: Adenosine Receptor A2a Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human A2AR (165-300/412aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: ADORA2A
• UniProt ID: P29274
• RRID: AB_10746263
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Adenosine Receptor A2a; Adenosine A2A-R; A2AR; A2A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Thymus (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: Thymus (Rat) Lysate at 40 ug, Lane 5: Lymph node (Rat) Lysate at 40 ug, Lane 6: SH-SY5Y (Human) cell Lysate at 30 ug, Lane 7: Molt-4 (Human) cell Lysate at 30 ug, Lane 8: U87MG (Human) cell Lysate at 30 ug, Lane 9: Huvec (Human) cell Lysate at 30 ug, Primary: Anti-Adenosine Receptor A2a (orb10012) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 47 kD.

Sample: MOlt4 (Human) Cell Lysate at 30 ug, Raji Cell Lysate at 30 ug, Primary: Anti-Adenosine Receptor A2a (orb10012) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 50 kD.

Sample: placenta (Mouse) Lysate at 40 ug, Primary: Anti-Adenosine Receptor A2a (orb10012) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45kD, Observed band size: 45kD.