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Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

Catalog Number: orb547931

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb547931
CategoryAntibodies
DescriptionAnti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityMonoclonal
Clone NumberC-1
Tested applicationsFC, ICC, IF, IHC, IP, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthesized peptide derived from human Actin (Alpha-Actin)
Antibody TypePrimary Antibody
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form/AppearanceLiquid
ConjugationUnconjugated
MW66 kDa
UniProt IDP68133
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesActin, alpha skeletal muscle; Alpha-actin-1; ACTA1
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NoteFor research use only
Application notesWB 1:1000-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Expiration Date12 months from date of receipt.
Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse muscle, using Actin (Alpha-Actin) Antibody (orb547931) ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (orb547931) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

Western blot analysis of Actin (Alpha-Actin) expression in (1) A431 cell lysate; (2) MCF-7 cell lysate (orb547931). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody (Catalog # orb547931) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

Anti-Actin (Alpha-Actin) ACTA1 Rabbit Monoclonal Antibody

IF analysis of immunocytochemical section of HeLa cells using anti-Actin (Alpha-Actin) antibody (orb547931). Actin (Alpha-Actin) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Actin (Alpha-Actin) Antibody (orb547931) overnight at 4 &deg.

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