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Catalog Number | orb547928 |
---|---|
Category | Antibodies |
Description | Anti-Actin ACTA1 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | BE-1 |
Tested applications | FC, ICC, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human Actin |
Antibody Type | Primary Antibody |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 66 kDa |
UniProt ID | P68133 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Actin, alpha skeletal muscle; Alpha-actin-1; ACTA1 Read more... |
Note | For research use only |
Application notes | WB 1:3000-1:20000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody.
Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) Human fetal kidney lysate; (3) 3T3 cell lysate; (4) Mouse spleen lysate; (5) PC-12 cell lysate; (6) Rat heart lysate.
Immunofluorescent analysis of HeLa cells, using Actin Antibody.
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human | |
Monoclonal | |
Unconjugated |