Anti-Actin ACTA1 Rabbit Monoclonal Antibody

• Catalog Number: orb547928
• Category: Antibodies
• Description: Anti-Actin ACTA1 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human Actin
• UniProt ID: P68133
• MW: 66 kDa
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Application notes: WB 1:3000-1:20000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: BE-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Actin, alpha skeletal muscle; Alpha-actin-1; ACTA1
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody.

Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) Human fetal kidney lysate; (3) 3T3 cell lysate; (4) Mouse spleen lysate; (5) PC-12 cell lysate; (6) Rat heart lysate.

Immunofluorescent analysis of HeLa cells, using Actin Antibody.