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Catalog Number | orb1906399 |
---|---|
Category | Antibodies |
Description | Anti-ABCG2 [5D3] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 5D3 |
Tested applications | FC, IF, IHC |
Reactivity | Human |
Isotype | IgG |
Immunogen | The original antibody was generated by immunizing mice with intact mouse 3T3 fibroblasts expressing the human ABCG2 protein. |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Target | ABCG2 |
UniProt ID | Q9UNQ0 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | CD338; CDw338; ABCP; BCRP; BCRP1; MXR; Breast canc Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence staining of A549 cells with anti-ABCG2 antibody 5D3. Immunofluorescence analysis of paraformaldehyde-fixed A549 cells on Shi-fix™ coverslips stained with the mouse IgG2b version of 5D3 (orb1906398) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906398, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256459) followed by staining with Alexa Fluor® 488 secondary antibody.