Anti-ABCG2 [5D3]

• Catalog Number: orb1906398
• Category: Antibodies
• Description: Anti-ABCG2 [5D3]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5D3
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: The original antibody was generated by immunizing mice with intact mouse 3T3 fibroblasts expressing the human ABCG2 protein.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: ABCG2
• UniProt ID: Q9UNQ0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD338; CDw338; ABCP; BCRP; BCRP1; MXR; Breast canc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of A549 cells with anti-ABCG2 antibody 5D3. Immunofluorescence analysis of paraformaldehyde-fixed A549 cells on Shi-fix™ coverslips stained with the mouse IgG2b version of 5D3 (orb1906398) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906398, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256459) followed by staining with Alexa Fluor® 488 secondary antibody.