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Anti-ABCG2 [5D3]
Description
Research Area
Images & Validation
−| Tested Applications | FC, IF, IHC |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | 5D3 |
| Immunogen | The original antibody was generated by immunizing mice with intact mouse 3T3 fibroblasts expressing the human ABCG2 protein. |
| Target | ABCG2 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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![Anti-ABCG2 [5D3]](/images/pub/media/catalog/product/NewWebsite/35/orb1906398_1.png)
Immunofluorescence staining of A549 cells with anti-ABCG2 antibody 5D3. Immunofluorescence analysis of paraformaldehyde-fixed A549 cells on Shi-fix™ coverslips stained with the mouse IgG2b version of 5D3 (orb1906398) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906398, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256459) followed by staining with Alexa Fluor® 488 secondary antibody.
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Protocol Information
Anti-ABCG2 [5D3] (orb1906398)
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