Annexin A1 Antibody

SKU: orb607106

Description

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Annexin A1 antibody to be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: ANXA1/1671
• Immunogen: Recombinant human full-length protein was used as the immunogen for the Annexin A1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human prostate with Annexin A1 antibody (clone ANXA1/1671). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human spleen with Annexin A1 antibody (clone ANXA1/1671). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Flow testing of fixed and permeabilized human HeLa cells with Annexin A1 antibody (clone ANXA1/1671). Red=isotype control, Blue= Annexin A1 antibody.

SDS-PAGE analysis of purified, BSA-free Annexin A1 antibody (clone ANXA1/1671) as confirmation of integrity and purity.

Western blot testing of human A549 cell lysate with Annexin A1 antibody (clone ANXA1/1671). Predicted molecular weight ~38 kDa.

Quick Database Links

UniProt

P04083