Annexin I Antibody

SKU: orb20132

Description

Goat polyclonal to Annexin I

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kDa according to NP_000691.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Paweletz et al , Cancer Res. 2000 Nov15;60(22):6293-7. PMID: 11103786). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Annexin I
• Molecular Weight: 38.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti annexin I antibody, anti ANXA1 antibody, anti annexin A1 antibody, anti ANX1 antibody, anti LPC1 antibody, anti annexin I (lipocortin I) antibody, anti lipocortin I antibody

Western blot analysis of A431 lysate using Annexin antibody

Western blot analysis of K562 (Lane 1) and NIH3T3 (Lane 2) lysates using Annexin I antibody

Immunohistochemical staining of Human Placenta using Annexin I antibody

Immunohistochemical staining of Human Prostate using Annexin I antibody

Western blot analysis of Rat Spleen lysate using Annexin I antibody

Western blot analysis of Pig Spleen lysate using Annexin I antibody

Quick Database Links

Gene Symbol

Annexin I

NCBI Gene ID

301