ANLN Antibody

SKU: orb1247798

Description

ANLN Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration: 3-10ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: WQPDACYKPIGKP
• Target: ANLN
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

ANLN, Scraps, ANILLIN, anillin, actin binding protein (scraps homolog, Drosophila), anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, DKFZp779A055, scra

orb1247798 (10 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247798 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining.

orb1247798 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

ANLN

UniProt

Q9NQW6

RefSeq (Protein):NP_001271230.1, NP_001271231.1, NP_061155.2