Goat anti-ANILLIN / Scraps (C Terminus) Antibody

• Catalog Number: orb18593
• Category: Antibodies
• Description: Goat polyclonal antibody to ANLN
• Target: ANILLIN / Scraps (C Terminus)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: WQPDACYKPIGKP
• RRID: AB_10749320
• MW: 124; 120; 120
• Tested applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:64000, IHC-P: 3-10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules.. Recommended concentration, 3-10ug/ml.WB: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ANLN antibody, anti Scraps antibody, anti ANI
• Note: For research use only
• Entrez: 54443

Immunohistochemical staining of Human Kidney using ANLN antibody

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells using ANLN antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using ANLN antibody