ANILLIN/Scraps (C Terminus) Antibody

SKU: orb18593

Description

Goat polyclonal antibody to ANLN

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:64000, IHC-P: 3-10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules.. Recommended concentration, 3-10ug/ml.WB: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ANILLIN / Scraps (C Terminus)
• Molecular Weight: 124; 120; 120
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ANLN antibody, anti Scraps antibody, anti ANILLIN antibody, anti anillin, actin binding protein (scraps homolog, Drosophila) antibody, anti anillin (Drosophila Scraps homolog), actin binding protein antibody, anti anillin, actin binding protein antibody, anti DKFZp779A055 antibody, anti scra antibody

Immunohistochemical staining of Human Kidney using ANLN antibody

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells using ANLN antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using ANLN antibody

Quick Database Links

Gene Symbol

ANILLIN / Scraps (C Terminus)

NCBI Gene ID

54443