Ambra1 Antibody

• Catalog Number: orb1239099
• Category: Antibodies
• Description: Ambra1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Equine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1.The immunogen is located within the last 50 amino acids of Ambra1.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 143kD Observed: 143 kD
• Target: AMBRA1
• UniProt ID: Q9C0C7
• NCBI: Q9C0C7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Ambra1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Ambra1 Antibody: DCAF3, WDR94, KIAA1736, Activatin
• Note: For research use only
• Application notes: WB: 1-2 μg/mL; IHC: 1-5 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunoflouorescence Validation of AMBRA1 in HeLa Cells. Immunofluorescent analysis of PFA-fixed HeLa cells labeling AMBRA1 with orb1239099 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

WB Validation in Human Cell Lines. Loading: 15 µg of lysate per lane Antibodies: AMBRA1 orb1239099, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in 3T3/NIH Cells. Loading: 15 µg of lysate Antibodies: AMBRA1 orb1239099, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of AMBRA1 in Rat Colon Tissue. Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-AMBRA1 antibody (orb1239099) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of AMBRA1 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling AMBRA1 with orb1239099 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) DAPI staining (blue).