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Catalog Number | orb377918 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to AMBRA1. |
Target | AMBRA1 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Immunogen | Recombinant full length protein of human AMBRA1 |
UniProt ID | A2AH22, Q9C0C7 |
Tested applications | IH, WB |
Dilution range | WB: 1:500-2000, IHC-P: 1:50-200 |
Antibody Type | Primary Antibody |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | KIAA1736; Activating molecule in BECN1-regulated a Read more... |
Note | For research use only |
Entrez | 228361, 55626 |
Western blot analysis of AMBRA1 expression in MCF7 (A), A431 (B) whole cell lysates. (Predicted band size: 84; 132; 135; 139; 142 kD; Observed band size: 143 kD)
Immunohistochemical analysis of AMBRA1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.