alpha Tubulin antibody

• Catalog Number: orb556566
• Category: Antibodies
• Description: Rabbit polyclonal antibody to alpha Tubulin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
• Concentration: 0.81 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Target: alpha Tubulin
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Note: For research use only
• Application notes: Positive control: NIH-3T3 , 293T , A431 , HeLa , HepG2
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A431 cells using alpha Tubulin antibody

Immunohistochemical staining of SW480 Xenograft using alpha Tubulin antibody

Western blot analysis of Various whole cell using alpha Tubulin antibody

Western blot analysis of NIH-3T3 cells using alpha Tubulin antibody