alpha Tubulin Rabbit Polyclonal Antibody

SKU: orb556566

Description

Rabbit polyclonal antibody to alpha Tubulin

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IHC-P, WB
• Dilution Range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Positive control: NIH-3T3 , 293T , A431 , HeLa , HepG2

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
• Target: alpha Tubulin
• Purity: Purified by antigen-affinity chromatography.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Buffer/Preservatives: PBS, 20% Glycerol, 0.025% ProClin 300.
• Concentration: 0.81 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescence analysis of A431 cells using alpha Tubulin antibody

Immunohistochemical staining of SW480 Xenograft using alpha Tubulin antibody

Western blot analysis of Various whole cell using alpha Tubulin antibody

Western blot analysis of NIH-3T3 cells using alpha Tubulin antibody

Quick Database Links

Gene Symbol

alpha Tubulin