Alpha Synuclein Monomers

SKU: orb1822363

Description

Human Recombinant Alpha Synuclein Monomers (Type 1)

Images & Validation

• Tested Applications: In vitro, In vivo, SDS-PAGE, WB
• Application Notes:
  Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL @ 2mg/mL.

Key Properties

• Source: Recombinant
• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: 100 µM alpha synuclein protein monomer seeded with 10 uM alpha synuclein PFFs in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
• Target: Alpha Synuclein Monomers
• Reactivity: Human
• Tag: No tag
• Molecular Weight: ~14.46 kDa
• Protein Length: Full Length
• Protein Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
• Purification: Ion-exchange Purified
• Purity: >95%

Storage & Handling

• Storage: -80°C
• Buffer/Preservatives: PBS pH 7.4
• Concentration: 2 mg/ml or 5 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson's disease familial 1 Protein

Type 1 alpha synuclein pre-formed fibrils seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of Type 1 alpha synuclein pre-formed fibrils is combined with 100 μM of alpha synuclein monomer, as compared to when 10 nM of Type 1 alpha synuclein pre-formed fibrils is combined with 100 μM of alpha Synuclein monomer. Thioflavin T ex = 450 nm, em = 485 nm.

SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer. Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 μg).

Quick Database Links

Gene Symbol

Alpha Synuclein Monomers

UniProt

P37840