Alpha Synuclein Monomers (Type 1)

• Catalog Number: orb1822361
• Category: Proteins
• Description: Mouse Recombinant Alpha Synuclein Protein Monomers (Type 1)
• Tested applications: In vitro, In vivo, SDS-PAGE, WB
• Reactivity: Mouse
• Tag: No tag
• Concentration: 2 mg/ml or 5 mg/ml
• Purity: > 95%
• MW: ~14.46 kDa
• Target: Alpha Synuclein
• UniProt ID: O55042
• Protein Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA
• Protein Length: Full Length
• Source: Recombinant
• Expression System: E. coli
• Biological Activity: 100 µM alpha synuclein protein monomer (SPR-323) seeded with 10 uM alpha synuclein protein PFF (SPR-324) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated an increased fluorescence intensity after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
• Storage: -80°C
• Buffer/Preservatives: PBS pH 7.4
• Alternative names: Alpha synuclein monomer, Alpha-synuclein monomer,
• Note: For research use only
• Application notes: Certified > 95% pure using SDS-PAGE analysis. Low endotoxin < 5 EU/mL @ 2mg/mL.
• Expiration Date: 6 months from date of receipt.

C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-Alpha Synuclein pSer129 at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.

Type 1 alpha synuclein pre-formed fibrils seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 μM of Type 1 alpha synuclein pre-formed fibrils is combined with 100 μM of alpha synuclein monomer, as compared to Type 1 alpha synuclein pre-formed fibrils or alpha synuclein monomer alone. Thioflavin T ex = 450 nm, em = 485 nm.

SDS-PAGE of ~14 kDa Mouse Recombinant Alpha Synuclein Protein Monomer. Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 μg).