Alpha Synuclein A53T Mutant Monomers

SKU: orb1822355

Description

Human Recombinant Alpha Synuclein A53T Mutant Monomers (Type 1)

Research Area

Neuroscience

Images & Validation

• Tested Applications: In vitro, In vivo, SDS-PAGE, WB
• Application Notes:
  Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL @ 2mg/mL.

Key Properties

• Source: Recombinant
• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: 100 µM A53T alpha synuclein protein monomer seeded with 10 uM A53T alpha synuclein protein PFF in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
• Target: Alpha Synuclein A53T Monomers
• Reactivity: Human
• Tag: No tag
• Molecular Weight: ~14.46 kDa
• Protein Length: Full Length
• Protein Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
• Purification: Ion-exchange Purified
• Purity: >95%

Storage & Handling

• Storage: -80°C
• Buffer/Preservatives: PBS pH 7.4
• Concentration: 2 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

A53T mutant alpha synuclein, A53T mutated SNCA, A53T Alpha synuclein monomer, Ala53thr mutant alpha synuclein, Alpha synuclein protein, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson disease familial 1 Protein

SDS-PAGE of ~14 kDa A53T Alpha Synuclein Monomer

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers. A much greater increase in fluorescence is seen when 100 uM monomer is combined with 10 nM of fibrils as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.

Quick Database Links

Gene Symbol

Alpha Synuclein A53T Monomers

UniProt

P37840