alpha smooth muscle Actin Rabbit mAb [KD Validated] Antibody

SKU: orb1951991

Description

Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: FC, ICC, IHC, WB
• Dilution Range: WB 1:1000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:20
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human alpha smooth muscle Actin
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46

All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody.

Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.

Western blot analysis of alpha smooth muscle Actin expression in (1) A549 cell lysate; (2) C2C12 cell lysate using alpha-SMA antibody.

Quick Database Links

UniProt

P62736