alpha smooth muscle Actin Rabbit mAb [KD Validated]

• Catalog Number: orb1951991
• Category: Antibodies
• Description: Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human alpha smooth muscle Actin
• UniProt ID: P62736
• Tested applications: FC, ICC, IHC, WB
• Dilution range: WB 1:1000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:20
• Antibody Type: Recombinant Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Actin, aortic smooth muscle, Alpha-actin-2, Cell g
• Note: For research use only

All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody.

Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.

Western blot analysis of alpha smooth muscle Actin expression in (1) A549 cell lysate; (2) C2C12 cell lysate using alpha-SMA antibody.