alpha smooth muscle Actin Rabbit mAb [KD Validated] Antibody

SKU: orb1951797

Description

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: FC, ICC, IHC, WB
• Dilution Range: WB 1:1000~1:5000 IHC 1:100~1:500 ICC/IF 1:100~1:500 FC 1:30
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human alpha smooth muscle Actin
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTA2; ACTSA; ACTVS; GIG46;

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using alpha smooth muscle Actin Antibody.

Western blot analysis on (1) A431 cell lysate; (2) C6 cell lysate using alpha-SMA antibody.

Quick Database Links

UniProt

P62736, P63267, P68032