alpha smooth muscle Actin Rabbit mAb [KD Validated]

• Catalog Number: orb1951797
• Category: Antibodies
• Description: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human alpha smooth muscle Actin
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:1000~1:5000 IHC 1:100~1:500 ICC/IF 1:100~1:500 FC 1:30
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P63267, P68032, P62736
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Actin; aortic smooth muscle; Alpha-actin-2; Cell g
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using alpha smooth muscle Actin Antibody.

Western blot analysis on (1) A431 cell lysate; (2) C6 cell lysate using alpha-SMA antibody.