Alpha Actin Antibody / ACTA2 (smooth muscle)

• Catalog Number: orb750255
• Category: Antibodies
• Description: Actin is a major component of the cytoskeleton and is present in most cell types. This mAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM332
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A 10 amino acid peptide from the alpha smooth muscle isoform of actin was used as the immunogen for this Smooth Muscle Actin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Smooth Muscle Actin antibody is available for research use only.
• UniProt ID: P62736
• Storage: Store the Smooth Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of fixed human HeLa cells with Smooth Muscle Actin antibody (clone SPM332, green) and NucSpot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded Leiomyosarcoma stained with Smooth Muscle Actin antibody (clone SPM332).