Smooth Muscle Actin Antibody

SKU: orb749297

Description

Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-4ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Variations in protocols, secondaries and substrates may require the Smooth Muscle Actin antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: 1A4
• Immunogen: An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth Muscle Actin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P62736