Alpha 1 Antitrypsin Antibody [AAT/1378]

• Catalog Number: orb1252343
• Category: Antibodies
• Description: Alpha 1 Antitrypsin Antibody [AAT/1378]
• Target: SERPINA1
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity
• Immunogen: A human partial recombinant protein was used as the immunogen for this Alpha 1 Antitrypsin antibody.
• UniProt ID: P01009
• Tested applications: FC, IF, IHC-P, WB
• Application notes: Western blot: 0.5-1 ug/mlIHC (Paraffin): 0.5-1 ug/mlIF: 0.5-1 ug/mlFlow Cytometry: 0.5-1ug/10e6 cells in 0.1mlThe stated application concentrations are suggested starting amounts. Titration of the Alpha 1 Antitrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Antibody Type: Primary Antibody
• Clone Number: AAT/1378
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Alpha-1-antitrypsin, Alpha-1 protease inhibitor, A
• Research Area: Cancer Biology, Cell Biology, Metabolism Research
• Note: For research use only

IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

Western blot testing of 1) a partial recombinant protein, 2) human Jurkat and 3) human A549 lysate with Alpha 1 Antitrypsin antibody (clone AAT/1378). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).

SDS-PAGE Analysis of Purified, BSA-Free Alpha 1 Antitrypsin Antibody (clone AAT/1378). Confirmation of Integrity and Purity of the Antibody.