ALG1 Antibody

• Catalog Number: orb1256163
• Category: Antibodies
• Description: ALG1 Antibody
• Target: ALG1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3).
• UniProt ID: Q9BT22
• MW: Observed: 60kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ALG1, HMAT1, HMT-1, HMT1, MT-1, CDG1K, Mat-1, hMat
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only

Western blot analysis of extracts of various cell lines, using ALG1 antibody (orb1256163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of A549 cells using ALG1 antibody (orb1256163). Blue: DAPI for nuclear staining.