AKT3 PE Antibody

SKU: orb344553

Description

AKT3 PE antibody (RPE)

Images & Validation

• Tested Applications: DOT, FC
• Dilution range: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Anti-AKT3 PE Antibody is tested for Flow Cytometry. This antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 9A5.H9.G7
• Immunogen: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
• Purity: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
• Conjugation: RPE

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: None
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Mouse anti-AKT3 antibody PE conjugation, phycoerythrin conjugated Mouse anti-AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

Dot Blot of AKT3 Phycoerythrin Conjugated Monoclonal Antibody. Antigen: AKT3 non-phosphorylated. Load: Lane 1: 50 ng. Lane 2: 16.67 ng. Lane 3: 5.56 ng. Lane 4: 1.85 ng. Lane 5: 0.62 ng. Primary antibody: none. Secondary antibody: AKT3 Phycoerythrin Conjugated Monoclonal Antibody at 1:1000 in orb348637 for 60 min at RT. Block: orb348637 for 60 min at RT.

Flow Cytometry of Mouse anti-AKT3 antibody. Cells: SK-OV3 Cells. Stimulation: none. Primary antibody: Phycoerythrin AKT3 antibody at 1.0 µg/ml for 20 min at 4°C.

Quick Database Links

UniProt

Q9Y243

RefSeq (Protein):NP_001193658.1